Detection, isolation and identification of more bioactive compounds from Fusarium equiseti, an endophytic isolated from Ocimum gratissimum

Endophytes have continued to gain fame due their ability produce an array of secondary metabolites within the host system with huge untapped pharmacological potentials. This study was carried out further identify and isolate novel therapeutic compounds from Fusarium equiseti, Endophytic fungus isolated leaves Ocimum gratissimum. fungal isolation, fermentation, extraction were using standard laboratory methods. The crude extracts equiseti subjected chromatographic techniques vacuum liquid chromatography, Sephadex LH 20 semipreparative HPLC for isolation bioactive compounds. fractions obtained high performance chromatography-Diode Array Detector (HPLC-DAD). analytical led detection many namely: Enniatin A, Aureonitol, Serasinoside H1, Altenusin, Aplysinamisin, benzylnitril, ruspolinone Orientin. Semipreparative 6 pure which two identified as ruspolinone. remaining four not lack library hits. detected been previously shown exhibit a wide biological activities including antiviral, antifungal, hepatoprotection, antibacterial, anticancer, cytotoxic, antioxidant properties. unidentified may hold enormous potential new lead development into agents. Therefore, fungus, should be harnessed its pharmacological, pharmaceutical, agricultural industrial applications.